[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].
Identifieur interne : 002D73 ( Main/Exploration ); précédent : 002D72; suivant : 002D74[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].
Auteurs : A A Bakova ; V A Gusev ; I A Popov ; A I Gordienko ; Iu L. Krivorutchenko ; Iu S. KrivosheinSource :
- Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) [ 1028-0987 ]
Descripteurs français
- KwdFr :
- Allantoïde (immunologie), Allantoïde (virologie), Animaux, Anticorps monoclonaux, Antigènes viraux (analyse), Chorion (immunologie), Chorion (virologie), Embryon de poulet, Humains, Hybridomes (immunologie), Hémagglutinines virales (immunologie), Protéines de la matrice virale (immunologie), Techniques immunoenzymatiques, Virus de la grippe A (immunologie).
- MESH :
- analyse : Antigènes viraux.
- immunologie : Allantoïde, Chorion, Hybridomes, Hémagglutinines virales, Protéines de la matrice virale, Virus de la grippe A.
- virologie : Allantoïde, Chorion.
- Animaux, Anticorps monoclonaux, Embryon de poulet, Humains, Techniques immunoenzymatiques.
English descriptors
- KwdEn :
- Allantois (immunology), Allantois (virology), Animals, Antibodies, Monoclonal, Antigens, Viral (analysis), Chick Embryo, Chorion (immunology), Chorion (virology), Hemagglutinins, Viral (immunology), Humans, Hybridomas (immunology), Immunoenzyme Techniques, Influenza A virus (immunology), Viral Matrix Proteins (immunology).
- MESH :
- chemical , analysis : Antigens, Viral.
- chemical , immunology : Hemagglutinins, Viral, Viral Matrix Proteins.
- chemical : Antibodies, Monoclonal.
- immunology : Allantois, Chorion, Hybridomas, Influenza A virus.
- virology : Allantois, Chorion.
- Animals, Chick Embryo, Humans, Immunoenzyme Techniques.
Abstract
The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.
PubMed: 8548071
Affiliations:
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Le document en format XML
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<term>Antigens, Viral (analysis)</term>
<term>Chick Embryo</term>
<term>Chorion (immunology)</term>
<term>Chorion (virology)</term>
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<term>Allantoïde (virologie)</term>
<term>Animaux</term>
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<term>Antigènes viraux (analyse)</term>
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<term>Chorion (virologie)</term>
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<term>Protéines de la matrice virale</term>
<term>Virus de la grippe A</term>
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<front><div type="abstract" xml:lang="en">The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.</div>
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<name sortKey="Gusev, V A" sort="Gusev, V A" uniqKey="Gusev V" first="V A" last="Gusev">V A Gusev</name>
<name sortKey="Krivorutchenko, Iu L" sort="Krivorutchenko, Iu L" uniqKey="Krivorutchenko I" first="Iu L" last="Krivorutchenko">Iu L. Krivorutchenko</name>
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