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[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].

Identifieur interne : 002D73 ( Main/Exploration ); précédent : 002D72; suivant : 002D74

[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].

Auteurs : A A Bakova ; V A Gusev ; I A Popov ; A I Gordienko ; Iu L. Krivorutchenko ; Iu S. Krivoshein

Source :

RBID : pubmed:8548071

Descripteurs français

English descriptors

Abstract

The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.

PubMed: 8548071


Affiliations:


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Le document en format XML

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<term>Antibodies, Monoclonal</term>
<term>Antigens, Viral (analysis)</term>
<term>Chick Embryo</term>
<term>Chorion (immunology)</term>
<term>Chorion (virology)</term>
<term>Hemagglutinins, Viral (immunology)</term>
<term>Humans</term>
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<term>Allantoïde (immunologie)</term>
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<term>Antigènes viraux (analyse)</term>
<term>Chorion (immunologie)</term>
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<term>Embryon de poulet</term>
<term>Humains</term>
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<term>Hémagglutinines virales (immunologie)</term>
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<div type="abstract" xml:lang="en">The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.</div>
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